Determination of the degree of contamination of the abdominal cavity and morphological characteristics of the liver in experimental animals during simulation of an abscess and after sanitation with antiseptics

Aim. To study the contamination of the abdominal cavity and the morphological characteristics of the liver in experimental animals when modeling an abscess and after sanitation with antiseptics.

Material and methods. Microbiological studies were carried out on 54 samples of pus and 54 samples of exudate, delivered from the cavity of the SMA, modeled in experimental experimental rabbits - male Chinchillas, to detect strains of microorganisms of the group of enterobacteria and microbes of the coccal flora. The rabbits were divided into 9 groups, each group consisting of 6 rabbits. The morphological characteristics of the liver of 24 rabbits were also studied during sanitation of the abdominal cavity with a solution of 0.1% furagin after 3.5 and 7 minutes of exposure. The 1st group included 8 intact rabbits that were not subjected to any experimental interventions, the 2nd group consisted of 8 rabbits in which an experimental abscess was created, but its sanitation was not carried out. The third group included 8 rabbits where a 0.1% furagin solution was used to sanitation an abscess with different exposure intervals: 3, 5 and 7 minutes.

Results. Studies regarding the growth of microbes after various exposures to sanitation with a 0.1% furagin solution have shown that the antiseptic solution of 0.1% furagin has a detrimental effect on the microbes of the abscess cavity and abdominal cavity, in particular, in an exposure of 5-7 minutes it practically destroys all existing microbes. And microscopic studies of liver tissue after a 7-minute exposure to the use of a 0.1% antiseptic solution of furagin did not reveal any changes.

Conclusion. A 0.1% solution of furagin has bactericidal properties, is the safest and has no toxic effect on liver tissue.

1. Gel’fand, B.R. Abdominal’naya hirurgicheskaya infekciya: Rossijskie nacional’nye rekomendacii [Abdominal surgical infection: Russian national recommendations]. 2-e izd., pererab. i dop. – M: OOO «Medicinskoe informacionnoe agentstvo», 2018, 168 s.: il..

2. Kulabuhov, V.V. Sepsis: kontrol’ ochaga infekcii [Sepsis: controlling the source of infection]. Vestnik anesteziologii i reanimatologii, 2021, No. 5(18), рр. 89-96.

3. Mikrobnyj pejzazh operirovannyh bol’nyh s ogranichennym steril’nym pankreonekrozom v razlichnye periody kompleksnogo lecheniya [Microbial landscape of operated patients with limited sterile pancreatic necrosis during various periods of complex treatment]. Byulleten’ VSNC SO RAMN, 2015, No. 2(102), рр. 99-102.

4. Mrugova, T.M. Serorezistentnost’ i ustojchivost’ k antibiotikam shtammov Escherichia coli, vydelennyh ot pacientov s infekcionno-vospalitel’nymi zabolevaniyami [Seroresistance and antibiotic resistance of Escherichia coli strains isolated from patients with infectious and inflammatory diseases]. Problemy medicinskoj mikologii, 2020, No.3(22), рр. 108.

5. Posleoperacionnye vnutribryushnye abscessy v gepatobiliarnoj hirurgii: voprosy vybora adekvatnogo metoda hirurgicheskogo lecheniya [Postoperative intra-abdominal abscesses in hepatobiliary surgery: issues of choosing an adequate method of surgical treatment]. Vestnik IPO v SZ RT, 2022, No. 4, рр. 30-39.

6. Effektivnost’ lecheniya peritonita u eksperimental’nyh zhivotnyh v zavisimosti ot sposoba sanacii bryushnoj polosti [The effectiveness of treatment of peritonitis in experimental animals depending on the method of sanitation of the abdominal cavity]. Medicinskij vestnik Severnogo Kavkaza, 2015, No. 4 (10), рр. 344-348.

7. Antibiotic sensitivity in correlation to the origin of secondary peritonitis: a single center analysis / R. Grotelüschen, L.M. Heidelmann, M. Lütgehetmann [et all.] // Scientific Reports, 2020, No. 1(10), рр. 1-9.

8. Characteristics and management of pyogenic liver abscess: A European experience / Serraino C. [et all.]. // Medicine (Baltimore), 2018, No. 97(19): e0628. doi: 10.1097/MD.0000000000010628. PMID: 29742700; PMCID: PMC5959441.

9. Clinical effects and prognostic significance of intra-abdominal pressure in secondary peritonitis / D. Mahmutaj, B. Braha, A. Hamza [et all.] // Journal of International Dental and Medical Research, 2021, No. 1(14), рр. 453-460.

10. Comparative analysis of percutaneous drainage versus operative drainage of intra-abdominal abscesses in a resourcelimited setting: The tanzanian experience / Ukweh O.N. [et all.] // Annals of global health, 2023, No. 89(1), рр. 1-7. DOI: https://doi.org/10.5334/aogh.4070.

11. Droniak M.M. Evaluation of the effectiveness of treatment of experimental postoperative peritonitis / M.M. Droniak // Journal of Education, Health and Sport, 2021, No. 11(1), рр. 290-296.

12. Epidemiology of intra-abdominal infection and sepsis in critically ill patients: «Abses», a multinational observational cohort study and ESICM Trials Group Project / S. Blot, M. Antonelli, K. Arvaniti [et al.] // Intensive care medicine, 2019, No. 12(45), рр. 1703-1717.

13. Microbial findings, sensitivity and outcome in patients with postoperative peritonitis a retrospective cohort study / R. Grotelueschen, M. Luetgehetmann, J. Erbes // International Journal of Surgery, 2019, Vol. 70, рр. 63-69.

14. Pyogenic liver abscess: Clinical features and microbiological profiles in tertiary care center / Kumar SK [et all.] // J. Family Med Prim Care, 2020, No. 9(8), рр. 4337-4342.

15. Population-Based study of unfavorable prognostic factors associated with pyogenic liver abscess / Bläckberg A [et all.] // Open Forum Infect Dis, 2023, No.10(8), ofad352. doi: 10.1093/ofid/ofad352. PMID: 37547858; PMCID: PMC10400158.